3.1.2.1 acetyl-CoA + H2O = CoA + acetate - - 3.1.2.1 acetyl-CoA + H2O = CoA + acetate as shown by proton nuclear magnetic resonance spectroscopy the disappearance of acetyl CH3 from acetyl Coenzyme A occurres concomitantly with the appearance of a CH3 peak corresponding to that of free acetate and suggesting that folate is not acetylated during the reaction 732790 3.1.2.1 acetyl-CoA + H2O = CoA + acetate as shown by proton nuclear magnetic resonance spectroscopy the disappearance of acetyl CH3 from acetyl Coenzyme A occurrs concomitantly with the appearance of a CH3 peak corresponding to that of free acetate and suggesting that folate is not acetylated during the reaction 732790 3.1.2.1 acetyl-CoA + H2O = CoA + acetate the bifunctional enzyme is identical with a 3-ketoacyl-CoA thiolase isozyme, i.e. THL, EC 2.3.1.9, determined by internal peptide amino acid sequence comparison 664215