5.3.2.6 (2Z,4E)-2-hydroxyhexa-2,4-dienedioate = (3E)-2-oxohex-3-enedioate - 5.3.2.6 (2Z,4E)-2-hydroxyhexa-2,4-dienedioate = (3E)-2-oxohex-3-enedioate catalytic reaction mechanism, detailed overview 5.3.2.6 (2Z,4E)-2-hydroxyhexa-2,4-dienedioate = (3E)-2-oxohex-3-enedioate catalytic reaction mechanism, detailed overview. Quantum mechanics/molecular mechanics simulation of proton transfer reaction and structure-function analysis, the ketonization mechanism in 4-OT depends on the enzymatic model used, detailed overview 5.3.2.6 (2Z,4E)-2-hydroxyhexa-2,4-dienedioate = (3E)-2-oxohex-3-enedioate substrate specificity and one-base molecular mechanism, overview