2.1.1.202 S-adenosyl-L-methionine + cytosine34 in tRNA precursor = S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNA precursor the enzyme forms a covalent enzyme-substrate intermediate with its target 736856 2.1.1.202 S-adenosyl-L-methionine + cytosine34 in tRNA precursor = S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNA precursor the formation of 5-methylcytosine34 is a strictly intron-dependent process 639471 2.1.1.202 S-adenosyl-L-methionine + cytosine40 in tRNA precursor = S-adenosyl-L-homocysteine + 5-methylcytosine40 in tRNA precursor the enzyme forms a covalent enzyme-substrate intermediate with its target 736856 2.1.1.202 S-adenosyl-L-methionine + cytosine40 in tRNA precursor = S-adenosyl-L-homocysteine + 5-methylcytosine40 in tRNA precursor the formation of 5-methylcytosine34 is a strictly intron-dependent process 639471 2.1.1.202 S-adenosyl-L-methionine + cytosine48 in tRNA = S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNA the enzyme forms a covalent enzyme-substrate intermediate with its target 736856 2.1.1.202 S-adenosyl-L-methionine + cytosine48 in tRNA = S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNA the formation of 5-methylcytosine48 is an intron-independent process 639471 2.1.1.202 S-adenosyl-L-methionine + cytosine49 in tRNA = S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNA the enzyme forms a covalent enzyme-substrate intermediate with its target 736856 2.1.1.202 S-adenosyl-L-methionine + cytosine49 in tRNA = S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNA the formation of 5-methylcytosine49 is an intron-independent process 639471