4.2.1.33 - 5658, 5659, 5660, 5663, 726596 4.2.1.33 nickel-affinity column chromatography and Superdex 200 gel filtration 729127 4.2.1.33 variants LeuD-1-156 and LeuD-1-168, by centrifugation, on Ni-NTA column and by gel filtration, more than 95% pure 701510