4.2.1.109 homogeneity, using the His-tag, the His-tag is cleaved with thrombin 691391 4.2.1.109 partial 2660 4.2.1.109 recombinant His-tagged enzyme residues 20-242 from Escherichia coli strain Rosetta 2(DE3) by metal affinity chhromatography, anion exchange chromatography, gel filtration, and ultrafiltration 728896