3.4.21.25	-	
3.4.21.25	40fold	
3.4.21.25	60% ammonium sulfate precipitation with 1.0:1.25 ratio of crude extract:t-butanol at pH and temperature of 8.0 and 20°C	
3.4.21.25	a quick purification method leads to an enzyme fraction with almost only the 67000 Da enzyme	
3.4.21.25	ammonium sulfate precipitation, DEAE-Sepharose column chromatography, and Superdex 200 gel filtration	
3.4.21.25	DEAE-Sepharose chromatography, CM-Sepharose chromatography and ammonium sulfate precipitation	
3.4.21.25	gel filtration, SDS-PAGE	
3.4.21.25	HiTrap chelating column chromatography and Superdex 200 gel filtration	
3.4.21.25	HiTrap SP column chromatography and Superdex 200 gel filtration	
3.4.21.25	Ni-NTA affinity column chromatography