3.1.4.38 - 95156, 95158, 95159, 95160, 95161, 95162 3.1.4.38 native enzyme from brain 396fold by heat treatment at 60°C, concanavalin A affinity chromatography, and hydroxylapatite chromatography, followed by dialysis, anion exchange chromatography and gel filtration 730472 3.1.4.38 partial 95144, 95146 3.1.4.38 recombinant C-terminally His6-tagged enzyme from Streptomyces lividans strain 1326 by ammonium sulfate fractionation, nickel affinity and hydrophobic interaction chromatography, native extracellular enzyme 53fold from culture supernatant by ammonium sulfate fractionation, dialysis, anion exchange chromatography, and ultrafiltration, followed by hydrophobic interaction chromatography, ultrafiltration, two different steps of anion exxchange chromatography, ultrafiltration, gel filtration, ultrafiltration, cation exchange chromatography, and again ultrafiltration 730076