3.1.13.1 - 134050, 134061, 682697 3.1.13.1 100fold 134057 3.1.13.1 200fold 134054 3.1.13.1 28.6fold 134055 3.1.13.1 30fold 134049 3.1.13.1 370fold, nearly to homogeneity 134058 3.1.13.1 5300-26500fold, to homogeneity 134063 3.1.13.1 all mutants, with the exception of R500K, by histidine affinity chromatography and the AKTA fast protein liquid chromatography system 709032 3.1.13.1 anion exchange chromatography at denaturing conditions, in some cases followed by isoelectric focusing and dye binding chromatography 666317 3.1.13.1 by affinity chromatography 700000