2.4.2.6 - 638040, 638041, 638046, 704429, 736587 2.4.2.6 ammonium sulfate precipitation and Sephacryl S-200 gel filtration 737137 2.4.2.6 Bio-Scale Mini UNOsphere Q column chromatography, and Superose 12 gel filtration 736846 2.4.2.6 DRTase I and DRTAse II 638051 2.4.2.6 preclearing of bacterial lysate with 40% ammonium sulfate and subsequent precipitation of proteins with 90% ammonium sulfate, gel filtration of dissolved protein on Sephacryl S-200 column, combination of fractions with N-deoxyribosyltransferase activity, precipitation of proteins with 90% ammonium sulfate, dialysis of dissolved proteins and concentration by ultrafiltration, second gel filtration on Sephacryl S-200 column, combination of active fractions and concentration by ultrafiltration, recovery of 70.4% of activity, storage at -20°C in 50% glycerol 691053 2.4.2.6 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography 759063 2.4.2.6 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration 759340 2.4.2.6 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and preparative gel fitration 758681 2.4.2.6 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and tag cleavage through thrombin 758927 2.4.2.6 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, method optimization for LaNDT 759530