2.3.1.24 - 675259 2.3.1.24 n-octyl beta-D-thioglucoside, DE-32, sphingosine-celite, Sepharose CL-6B 486668 2.3.1.24 native enzyme 98.7fold from liver mitochondrionrich fraction by solubilization with n-octyl beta-D-thioglucoside, ion exchange chromatography, sphingosine affinity chromatography, and gel filtration 754575 2.3.1.24 recombinant His-tagged LASS5 from MLE cells by nickel affinity chromatography and ultrafiltration 754360 2.3.1.24 solubilization of membrane-bound enzyme from brain mitochondria by 2% of Trinton X-100, followed by ammonium sulfate fractionation and dialysis 752899 2.3.1.24 subcellular fractionation of HeLa cells recombinantly expressing the CerS isozyme 753223