2.3.1.18 affinity chromatography 486463 2.3.1.18 K12 strain H30000 486464 2.3.1.18 recombinant His-tagged enzyme from Escherichia coli strain BL21-CodonPlus(DE3)RIPL by nickel affinity chromatography, tag cleavage with TEV protease, another step of nickel affinity chromatography, and gel filtration 736706 2.3.1.18 recombinant His6-tagged wild-type and mutant enzymes by nickel affinity chromatography, ion exchange chromatography, and gel filtration 672425 2.3.1.18 strain ML308 486460, 486461, 486464 2.3.1.18 using a HiTrap metal-chelating affinity chromatography column, a HiTrap desalting, and a Superdex 200 column 672425 2.3.1.18 wild-type and mutant enzyme 486466