2.3.1.122	ammonium sulfate fractionation, gel filtration and Sephadex column chromatography	486242	
2.3.1.122	native enzyme from strain BCG, recombinant enzyme from Escherichia coli	673960	
2.3.1.122	recombinant proteins purified by affinity chromatography on Ni2+ charged His-trap columns	486244	
2.3.1.122	using Ni-NTA chromatography	702229, 704439	
2.3.1.122	using Ni-NTA chromatography and by applying a linear imidazole gradient from 10 to 500 mM. The final purification step contains gel filtration on a Superdex 200 column	705100