1.3.1.44 - 390806, 390807, 656287 1.3.1.44 native enzyme 1687fold to homogeneity in several steps, recombinant His-tagged enzyme from Escherichia coli by affinity chromatography 656287 1.3.1.44 Ni-NTA agarose column chromatography 686722 1.3.1.44 partial 390808 1.3.1.44 purified by gel-filtration chromatography on a HiLoad 16/60 superdex 200 column 723850 1.3.1.44 using Ni-NTA chromatography 724240