1.3.1.2	-	
1.3.1.2	ammonium sulfate precipitation, DEAE-cellulose column chromatography, and 2',5'-ADP Sepharose-4B column chromatography	
1.3.1.2	partial	
1.3.1.2	recombinant protein	
1.3.1.2	recombinant wild-type and mutant enzymes from Escherichia coli by affinity chromatography and gel filtration	
1.3.1.2	wild-type and mutant enzyme