1.14.13.81 cells are centrifuged, sonicated, cell debris removed by centrifugation, membrane fraction separated from the soluble fraction by ultracentrifugation, chlorosome fractioned by sucrose density gradient (15-45%), for SDS-PAGE chlorosome fraction is incubated in methanol, centrifuged, the pellet containing the chlorosome envelop is subjected to SDS-PAGE, protein identification by MALDI-TOF and liquid chromatography-tandem mass spectrometry 698617 1.14.13.81 partial 636329 1.14.13.81 partial, soluble fraction 636330 1.14.13.81 partially by chloroplast supernatant and membrane preparation of wild-type and recombinant mutant plants 676791 1.14.13.81 single-subunit O2-dependent cyclase AcsF, recombinant N-terminally His6-tagged enzyme from Escherichia coli strain C43(DE3) by nickel affinity chromatography and gel filtration 765462