1.14.11.48	purified from both the fungal mycelium and recombinant Escherichia coli cells	
1.14.11.48	recombinant protein, the nickel affinity purified protein is subjected to ion-exchange chromatography once the N-terminal 6XHis-tag has been successfully removed, followed by size-exclusion purification	
1.14.11.48	wild-type enzyme variants are purified from recombinant Escherichia coli cells