1.13.11.4 - 1.13.11.4 ammonium sulfate fractionation, DEAE-Sepharose fast flow column chromatography 1.13.11.4 GSTrap fast flow column chromatography 1.13.11.4 His-Bind protein chromatography 1.13.11.4 HiTrapQ column chromatography 1.13.11.4 Ni-NTA column chromatography 1.13.11.4 nickel-nitriloacetic acid resin column chromatography 1.13.11.4 partial 1.13.11.4 recombinant 1.13.11.4 recombinant enzyme