7.2.4.4 isolation of the biotinyl protein from the complex in catalytically inactive form in presence of detergent 684744 7.2.4.4 purification of subunit acetyl-S-acyl carrier protein: malonate acyl carrier protein-SH transferase and of whole enzyme complex 684745 7.2.4.4 recombinant subunit biotinyl protein MadF. Despite coexpression of biotin ligase birA, MadF is poorly biotinylated 684746