6.3.2.49 - 662037, 729244, 730896 6.3.2.49 His Trap 702771 6.3.2.49 recombinant His-tagged enzyme from Escherichia coli 690733 6.3.2.49 recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration 693286 6.3.2.49 recombinant N-terminally GST-tagged wild-type and mutant enzymes (deletion mutant comprising residues Lys4-Tyr468) from Escherichia coli strain ArcticExpress RIL cells (DE3) by glutathione affinity chromatography, tag cleavage by PreScission protease, dialysis and removal of the tag by glutathione affinity chromatography, followed by ultrafiltration and gel filtration, recombinant selenomethionine-labeled enzyme from Escherichia coli strain B834(DE3) purified by the same procedure 726615 6.3.2.49 recombinant wild-type enzyme and selenomethionine-labeled enzyme from Escherichia coli by anion exchange and hydrophobic interaction chromatography, followed by another step of anion exchange chromatography and gel filtration 726581