3.5.4.16 - 209541, 209544, 209548, 209549, 209550, 209552, 209553, 209554, 209558, 209559, 209561, 209564, 209566, 712290, 755735, 756001 3.5.4.16 ammonium sulfate precipitation, Ni-NTA column chromatography, and Sephacryl S-300HR gel filtration 698830 3.5.4.16 expression in Escherichia coli 209556, 209557 3.5.4.16 expression of wild-type and mutant proteins in Escherichia coli 209562 3.5.4.16 GCH1 in complexes with cellular proteins from organs liver, heart, brain, and kidney, by immunoaffinity chromatography, isolation of mitochondria from liver, heart and kidney 720858 3.5.4.16 Mono Q HR column chromatography 685163 3.5.4.16 Ni-NTA resin column chromatography 687523 3.5.4.16 Ni2+-Sepharose column chromatography, and gel filtration 734606 3.5.4.16 recombinant DELTA42-hGTP-CH-I from Escherichia coli M15 657176 3.5.4.16 recombinant enzyme from Escherichia coli strain BL21(DE3) by ion exchange chromatography 669149