3.4.23.48 purification of a His6-tagged fusion protein from Escherichia coli and reconstitution with lipopolysaccharide to an enzymatically active form. Purified His-tagged enzyme is coated onto fluorescent micro-particles and expresses plasminogen activity. The enzyme molecules have intrinsic adhesive properties. The purified transmembrane proteins coated onto fluorescence micro-particles can be used for functional assays 668672 3.4.23.48 recombinant plasminogen activator Pla, gel filtration 683251 3.4.23.48 recombinant protein, gel fitration 683853