3.4.22.43 - 137272 3.4.22.43 gel filtration, pre-activated before use 683662 3.4.22.43 gel filtration, recombinant human cathepsin V 683210 3.4.22.43 Ni-NTA column chromatography 732867 3.4.22.43 recombinant enzyme from Pichia pastoris to homogeneity, activated mature native enzyme by hyrophobic interaction chromatography, recombinant GST-tagged enzyme from Escherichia coli by glutathione affinity chromatography 668763 3.4.22.43 recombinant enzyme from Pichia pastoris, further purified by gel filtration after activation by pepsin treatment 669257