3.4.22.33 - 30273, 30274, 30296, 30299, 710318 3.4.22.33 2 forms: FBA and FBB 30278 3.4.22.33 bromelain from pineapple stem and skin are recovered by a PEG 4000/phosphate aqueous two-phase systems (ATPs) liquid-liquid extraction 717487 3.4.22.33 CM-Sepharose column chromatography, SP-Sepharose column chromatography, and Sephadex G-50 gel filtration 682716 3.4.22.33 fractionation 30277, 30278 3.4.22.33 fruit bromelain A, B and C 30277 3.4.22.33 high-speed counter-current chromatography coupled with the reverse micelle solvent system consisting of 0.10 g/ml cetyltrimethylammonium bromide (CTAB)/isooctane-hexylalcohol and 0.05 M sodium phosphate buffer is successfully applied to separate bromelain from pineapple fruit 717625 3.4.22.33 native enzyme 2.24fold 753642 3.4.22.33 native enzyme 2.88fold 753642 3.4.22.33 native enzyme 7.23fold from fruits by ammonium sulfate fractionation, dialysis, and anion exchange chromatography 754309