3.4.16.6 archieved by a two-step chromatographic process using cation-exchange, SP Sepharose, followed by anion-exchange, DEAE Sepharose FF resin 701056 3.4.16.6 domain 1B and 2 expressed in Sf9 cells using baculovirus expression system 647241 3.4.16.6 KEX1 gene from Saccharomyces cerevisiae expressed using the baculovirus/insect cell system 647238 3.4.16.6 using anion-exchange chromatography and gel filtration 731176 3.4.16.6 using IgG-Sepharose 698771