3.2.2.8 - 137116, 654131, 657373, 724342 3.2.2.8 Ni-NTA column chromatography 700741 3.2.2.8 Ni-NTA column chromatography and MonoQ column chromatography 696258 3.2.2.8 partial purification, 300fold 137117 3.2.2.8 recombinant enzyme 679803 3.2.2.8 recombinant enzyme from strain W6 by nickel chelate affinity chromatography and gel filtration 664140 3.2.2.8 recombinant His6-tagged enzyme from strain BL21(DE3) by nickel chelate affinity chromatography and gel filtration, the His-tag is cleaved off by thrombin 663495