2.7.4.25 - 2.7.4.25 anionic-exchange column chromatography, gel filtration, and anionic-exchange resin column chromatography 2.7.4.25 blue Sepharose column chromatography and Ultrogel AcA54 gel filtration 2.7.4.25 Ni-NTA column chromatography 2.7.4.25 of the recombinant protein 2.7.4.25 of the recombinant proteins