2.3.1.47 - 755855 2.3.1.47 from Escherichia coli DH5alpha, ammonium sulfate fractionation, gel filtration, hydrophobic interaction chromatography, ion exchange chromatography 718696 2.3.1.47 Glutathione Sepharose chromatography, GST-tag cleavage, Glutathione Sepharose chromatography 719127 2.3.1.47 Glutathione Sepharose chromatography, GST-tag cleavage, Glutathione Sepharose chromatography, gel filtration 719127 2.3.1.47 Glutathione Sepharose chromatography, GST-tag cleavage, Glutathione Sepharose chromatography, heat treatment with centrifugation 719127 2.3.1.47 Glutathione Sepharose chromatography, GST-tag cleavage, Glutathione Sepharose chromatography, ion exchange chromatography 719127 2.3.1.47 immobilized metal ion affinity chromatography (Ni2+), His-tag cleavage, immobilized metal ion affinity chromatography (Ni2+), gel filtration 719127 2.3.1.47 maltose binding protein affinity chromatography 720623 2.3.1.47 native enzyme partially by subcellular fractionation, recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration 676580 2.3.1.47 Ni-NTA column chromatography 755855