2.3.1.135 affinity labeling with all-trans-retinyl-alpha-bromoacetate, SDS electrophoresis, prior to affinity labelling, incubation with cholesterol chloroacetate to block nucleophilic reagents, or streptavidin column to remove endogenously biotinylated proteins enhance protein purification 638617, 638625 2.3.1.135 affinity labeling with N-Boc-L-biocytinyl-11-aminoundecan chloromethylketone, precipitation and gel electrophoresis, prior to affinity labelling, incubation with cholesterol chloroacetate to block nucleophilic reagents, or streptavidin column to remove endogenously biotinylated proteins enhance protein purification 638617, 638625 2.3.1.135 enzyme transfected in HEK-293T cells partially purificated by solubilization and centrifugation 638621 2.3.1.135 histidine-tagged truncated LRAT 685367 2.3.1.135 labeling with N-Boc-L-biocytinyl-11-aminoundecan chloromethylketone, affinity chromatography, avidin, prior to affinity chromatography or affinity labelling, incubation with cholesterol chloroacetate to block nucleophilic reagents, or streptavidin column to remove endogenously biotinylated proteins enhance protein purification 638617, 638625 2.3.1.135 native enzyme partially by membrane preparation 672075 2.3.1.135 native enzyme partially by membrane preparation from retinal pigment epithelium 674833 2.3.1.135 native enzyme partially by microsome preparation from eyes, recombinant enzyme partially from HEK-293T cells by membrane preparation 658662 2.3.1.135 recombinant His-tagged LRAT by affinity chromatography 720353 2.3.1.135 recombinant His-tagged truncated wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography in presence of 1% SDS 658075