2.1.1.204 - 710547 2.1.1.204 recombinant GST-tagged enzyme from Escherichia coli strain BL21(DE3) by ultracentrifugation, glutathione affinity chromatography, and tag cleavage through PreScission protease, followed by heparin affinity chromatography, gel filtration, and ultrafiltration 758443 2.1.1.204 recombinant His-tagged enzyme from Escherichia coli strain Rosetta2 (DE3) by nickel affinity chromatography and dialysis 756078 2.1.1.204 recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) 735726 2.1.1.204 recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli to over 90% purity, except for mutant R84A, which is about 75% pure 718931