1.8.4.8	-	
1.8.4.8	HiTrap chelating column chromatography and Superdex 200 gel filtration	
1.8.4.8	recombinant enzyme from strain BL21(DE3)	
1.8.4.8	recombinant His-tagged enzyme from strain BL21(DE3) by nickel affinity chromatography	
1.8.4.8	recombinant His10-tagged enzyme from strain BL21(DE3) by chelate affinity chromatography