1.3.8.12 - 730403 1.3.8.12 HisTrap column chromatography and Superdex 200 gel filtration 763257 1.3.8.12 recombinant His-tagged wild-tpye and mutant enzymes from Escherichia coli strain Rosetta pLys (DE3) by nickel affinity chromatography of cell-free supernatant, desalting gel filtration, tag cleavage by thrombin, gel filtration, and ultrafiltration 763257 1.3.8.12 recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain Rosetta (DE3) pLysS by nickel affinity chromatography and ultrafiltration 763481