1.14.18.3 - 659033, 660351, 702170 1.14.18.3 by centrifugation and gel filtration 705192 1.14.18.3 DEAE-Sepharose Fast Flow column chromatography, lysine agarose column chromatography, Sephacryl S-300HR gel filtration, QEA-Sephadex A-50 column chromatography, and Sephacryl S200 gel filtration, Superdex 200 gel filtration, or ammonium sulfate precipitation followed by Source 30Q column chroamtography 716108 1.14.18.3 FPLC liquid chromatography and Mono Q HR 5/50 GL column chromatography 690034 1.14.18.3 membrane-associated methane-oxidizing complex consisting of the particulate methane mono-oxygenase, pMMOH, and an unidentified component, assigned as a potential particulate methane mono-oxygenase reductase, pMMOR 657810 1.14.18.3 MonoQ 10/100 GL column chromatography and Sephacryl S100 gel filtration 728079 1.14.18.3 native enzyme from mebranes by solubilization with n-dodecyl beta-D-maltopyranoside, anion exchange chromatography, and ultrafiltration 745305 1.14.18.3 native holoenzyme from isolated membranes by solubilization with detergent n-dodecyl beta-D-maltoside, ultrafiltration, and gel filtration 744325 1.14.18.3 Ni2+-Sepharose Fast Flow column chromatography 685113 1.14.18.3 optimization of solubilization and purification procedure for the hydroxylase component of membrane-bound enzyme, purification to homogeneity involves solubilization by dodexylbeta-D-maltoside, ion exchange chromatography and gel filtration, the purification includes the loss of the reductase component 672301