1.14.14.3 - 348579, 348582, 348594, 348599, 348604, 348606, 348607, 348608 1.14.14.3 affinity methods 348560 1.14.14.3 DEAE-Sepharose column chromatography, Superdex 200 gel filtration, and Mono Q column chromatography 728435 1.14.14.3 HPLC 348561 1.14.14.3 nickel affinity column 698992 1.14.14.3 on a nickel affinity column, to more than 90% purity 702318 1.14.14.3 on nickel affinity column, to more than 90% purity 704579 1.14.14.3 preparation of enzyme with modified subunits 348584 1.14.14.3 preparation of subunits 348583 1.14.14.3 recombinant enzyme from Escherichia coli 658147