1.1.3.5 - 1.1.3.5 ammonium sulfate, DEAE-cellulose, Sephadex G-200 1.1.3.5 DEAE-Sepharose, Sephacryl S-200, phenyl-Sepharose, chromatofocusing, native and recombinant enzyme 1.1.3.5 native enzyme by ammonium sulfate fractionation, followed by anion exchange chromatography and gel filtration 1.1.3.5 partially 1.1.3.5 perfusion chromatography (Poros DEAE-50, Poros HP2, Poros HQ), Sephacryl S200 1.1.3.5 Q-Sepharose, Phenyl-sepharose, Superdex 200