1.1.2.4 - 1.1.2.4 by affinity chromatography 1.1.2.4 by centrifugation, sonication and on Ni2+-NTA column, to near homogeneity 1.1.2.4 by fast protein liquid chromatography on hydroxylapatite and on AH-Sepharose with covalently bound 4-hydroxy-a cyanocinnamic acid 1.1.2.4 hydroxylapatite column chromatography and 4-hydroxy-alpha-cyanocinnamic acid-AH-Sepharose column chromatography