1.1.1.3 separation of the two isozymes using Blue-Sepharose chromatography 246382 1.1.1.3 separation of the two isozymes using Matrex Gel Red A affinity chromatography 246382 1.1.1.3 threonine sensitive and threonine insensitive isozymes 246385 1.1.1.3 partial 246393 1.1.1.3 - 246383, 246384, 246388, 246389, 246391, 246392, 246395, 246398, 246399 1.1.1.3 overexpressed in Escherichia coli 246402 1.1.1.3 recombinant separated catalytic domains 654640 1.1.1.3 recombinant enzyme 662432, 712490 1.1.1.3 native enzyme partially from seeds by ammonium sulfate fractionation and desalting gel filtration 689647 1.1.1.3 recombinant His-tagged wild-type and mutant enzymes from Echerichia coli strain Rosetta (DE3) pLysS by nickel affinty chromatography and gel filtration 739791