1.1.1.290 metal-chelate chromatography on Ni-NTA resin and size exclusion chromatography 667077 1.1.1.290 Ni-agarose column chromatography 724295 1.1.1.290 recombinant His-tagged PdxB from Escherichia coli stran BL21(DE3) by nickel affinity chromatography and ultrafiltration 724295