5.4.2.8 - 3288, 3349, 3350, 3352, 3355, 680450, 690999, 749116 5.4.2.8 ammonium sulfate precipitation, ion exchange column chromatography, and Sephacryl S-100 gel filtration 748602 5.4.2.8 ammonium sulfate, Sephadex G-25, KM-cellulose, partially purified 649781 5.4.2.8 cell extracts are purified by His-tag agarose beads 705202 5.4.2.8 DEAE-Sepharose column chromatography and Superdex 75 gel filtration 748183 5.4.2.8 heat treatment 85°C, 20 min, anion-exchange, hydrophobic and gel filtration column chromatography 662018 5.4.2.8 Ni-NTA column chromatography 693184 5.4.2.8 Ni-Sepharose column chromatography 715129 5.4.2.8 nickel affinity chromatography column chromatography 714628 5.4.2.8 nickel-affinity column chromatography 662065 5.4.2.8 nickel-chelating resin chromatography 692918 5.4.2.8 partial 3357 5.4.2.8 partial, alginate-producing strain V388 3353 5.4.2.8 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography 727228 5.4.2.8 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3), removal of the His-tag, purification by nickel affinity chromatography and dialysis 727988 5.4.2.8 recombinant His6-tagged enzyme from Escherichia coli by nickel affinity chromatography and gel filtration, removal of the His-tag by TEV protease 728329 5.4.2.8 recombinant PGM 650233 5.4.2.8 recombinant PMM/PGM 649138 5.4.2.8 recombinant PMM2, polyethylene glycol, DEAE-Sepharose, Q-Sepharose 649661 5.4.2.8 Talon metal affinity resin chromatography 693211