5.3.2.6 - 721792, 747133, 748742 5.3.2.6 DEAE-Sepharose column chromatography and SP-Sepharose column chromatography 726816 5.3.2.6 native enzyme from Escherichia coli strain C by ammonium sulfate fractionation, gel filtration, anion exchange chromatography, ultrafiltration, ammoniums ulfate fractionation, and hydrophobic interaction chromatography, followed by gel filtration 719634 5.3.2.6 phenyl-Sepharose column chromatography and DEAE-Sepharose column chromatography 726964 5.3.2.6 recombinant enzyme from Escherichia coli by ultracentrifugation, two different steps of anion exchange chromatography, hydroxylapatite and hydrophobic interaction chromatography, and gel filtration with alternating steps of ultrafiltration 719633 5.3.2.6 recombinant hh4-OT wild-type and mutants from Escherichia coli by heat treatment, anion exchange chromatography, and gel-filtration 718874 5.3.2.6 recombinant wild-type and mutant enzymes from Escherichia coli strain BL21-Gold(DE3) to over 95% purity 718844 5.3.2.6 recombinant wild-type and mutant YwhBs from Escherichia coli strain BL21(DE3)pLysS 718847 5.3.2.6 recombinant wild-type and mutants from Escherichia coli strain BL21(DE3)pLysS to homogeneity 718830