5.1.3.1	-	
5.1.3.1	lysate is applied to a chelating sepharose fast flow column charged with Ni2+, the N-terminal His tag is removed by thrombin cleavage	
5.1.3.1	mutant enzymes D186A, D186N, D186E	
5.1.3.1	Ni-affinity column chromatography and Superdex G75 gel filtration	
5.1.3.1	partial	
5.1.3.1	recombinant cytosolic enzyme	
5.1.3.1	recombinant His-tagged enzyme	
5.1.3.1	recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and diaylsis