4.2.1.109	homogeneity, using the His-tag, the His-tag is cleaved with thrombin	
4.2.1.109	partial	
4.2.1.109	recombinant His-tagged enzyme residues 20-242 from Escherichia coli strain Rosetta 2(DE3) by metal affinity chhromatography, anion exchange chromatography, gel filtration, and ultrafiltration