3.6.5.4 - 644163, 644165, 644166, 644168, 644169, 644171, 644172, 644173, 644175, 644180, 657913, 658538, 660000, 660347, 660353, 669585, 686396, 686752, 687902, 687905, 688395, 690025 3.6.5.4 Ffh NG is purified 684156 3.6.5.4 Ffh NG is purified, the Ffh NG-FtsY NGd20 complex is purified using ion exchange chromatography, a Q Sepharose and a SP Sepharose column 688692 3.6.5.4 FtsY NGd20 is purified, the Ffh NG-FtsY NGd20 complex is purified using ion exchange chromatography, a Q Sepharose and a SP Sepharose column 688692 3.6.5.4 glutathione Sepharose column chromatography 696594, 696602, 699552 3.6.5.4 heat selective precipitation, cobalt-chelating affinity chromatography, gel filtration, and ion-exchange chromatography 700888, 700889 3.6.5.4 HiPrep heparin Sepharose column chromatography, SP Sepharose column chromatography, and Superdex 75 gel filtration 695328 3.6.5.4 Ni-Sepharose column chromatography 700263 3.6.5.4 proteins are purified using histidine-binding magnetic agarose beads 684117 3.6.5.4 recombinant 669412 3.6.5.4 recombinant C-terminally His6-tagged wild-type and mutant mature cpSRP54 (residues 76-564) from Escherichia coli strain BL21 Star 756198 3.6.5.4 recombinant His-tagged chimeric enzyme constructs from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography,anion exchange chromatography, and cation exchange chromatography, followed by gel filtration 757758 3.6.5.4 recombinant His-tagged chimeric enzyme constructs from Escherichia coli strain BL21(DE3) 757758 3.6.5.4 recombinant His-tagged chimeric enzyme constructs from Escherichia coli strain BL21(DE3) by nickel affinity chromatography,anion exchange chromatography, and cation exchange chromatography, followed by gel filtration 757758 3.6.5.4 recombinant His6-tagged signal recognition particle protein cpSRP54 from Escherichia coli strain Rosetta by nickel affinity chromatography, cation exchange chromatography, and anion exchange chromatography 757189 3.6.5.4 recombinant wild-type and mutant cpSRP54 from Escherichia coli strain Rosetta BL21 by two steps of cation exchange chromatography 718911 3.6.5.4 the FtsY NGd20 protein is purified over a HiTrap Blue column, desalted, then passed over a HiTrap SP and a HiTrap Q Sepharose column 689926