3.5.4.16 - 209541, 209544, 209548, 209549, 209550, 209552, 209553, 209554, 209558, 209559, 209561, 209564, 209566, 712290, 755735, 756001 3.5.4.16 ammonium sulfate precipitation, Ni-NTA column chromatography, and Sephacryl S-300HR gel filtration 698830 3.5.4.16 expression in Escherichia coli 209556, 209557 3.5.4.16 expression of wild-type and mutant proteins in Escherichia coli 209562 3.5.4.16 GCH1 in complexes with cellular proteins from organs liver, heart, brain, and kidney, by immunoaffinity chromatography, isolation of mitochondria from liver, heart and kidney 720858 3.5.4.16 Mono Q HR column chromatography 685163 3.5.4.16 Ni-NTA resin column chromatography 687523 3.5.4.16 Ni2+-Sepharose column chromatography, and gel filtration 734606 3.5.4.16 recombinant DELTA42-hGTP-CH-I from Escherichia coli M15 657176 3.5.4.16 recombinant enzyme from Escherichia coli strain BL21(DE3) by ion exchange chromatography 669149 3.5.4.16 recombinant HAT-tagged enzyme from Escherichia coli, 6.8fold 657273 3.5.4.16 recombinant His-tagged wild-type GTPCH maltose-binding protein fusion protein from Escherichia coli by amylose affinity chromatography and cleavage of the fusion tag by TEV protease, purification of His-tagged DELTA45-GTPCH mutant from HEK-293 cells by metal affinity chromatography 719914 3.5.4.16 recombinant maltose binding protein fusion enzyme fromn Escherichia coli strain DH5alpha 669268 3.5.4.16 recombinant wild-type and mutant enzymes 655934 3.5.4.16 two forms 209560