3.4.23.23 - 3.4.23.23 ammonium sulfate precipitation, DEAE-52 column chromatography and Sephadex G-100 gel filtration 3.4.23.23 HiTrap column chromatography 3.4.23.23 partially purified by 30% (w/v) polyethylene glycol/ 13.2% (w/v) sodium sulfate aqueous two-phase systems 3.4.23.23 purification by direct extraction of a fermentation system