3.4.23.18 - 30556, 30560, 30565, 649170, 652904 3.4.23.18 acetone precipitation 754494 3.4.23.18 concanavalin A-agarose column chromatography 752341 3.4.23.18 cytoplasmic enzyme: partial 30570 3.4.23.18 from crude powder preparation 30567 3.4.23.18 from koji culture and submerged cell culture 30564 3.4.23.18 from Molsin, a commercial crude enzyme extract 30557 3.4.23.18 HisTrap column chromatography and Superdex G-75 gel filtration 753450 3.4.23.18 Ni2+-Sepharose column chromatography and glutathione Sepharose column chromatography 755227 3.4.23.18 partial 30561 3.4.23.18 recombinant enzyme from Escherichia coli after solubilization from inclusion bodies by ion exchange chromatography to homogeneity 668812 3.4.23.18 several isoforms (cytoplasmic F1 and F2) 30570 3.4.23.18 several isoforms (extracellular A1 and A2) 30569, 30570 3.4.23.18 several isoforms (membrane-bound M1 and M2) 30575 3.4.23.18 the enzyme is purified by 46fold from an Amano protease preparation from Aspergillus oryzae 754932