3.4.22.53 E-F hand structure-domains 643971 3.4.22.53 large-scale 643983 3.4.22.53 - 643980, 643982, 643987, 644027, 644030, 644045, 644046, 644052 3.4.22.53 recombinant wild-type and mutant enzymes 644019 3.4.22.53 wild-type and mutant calpains 644019 3.4.22.53 recombinant enzyme 643968, 644028, 644029 3.4.22.53 m-calpain 669062 3.4.22.53 DEAE-Sepharose column chromatography 707559 3.4.22.53 ammonium sulfate precipitation, DEAE-cellulose column chromatography, phenyl-Sepharose column chromatography, Reactive Red column chromatography, and DEAE-TSK anion exchange column chromatography 708954 3.4.22.53 ammonium sulfate precipitation, DEAE-cellulose column chromatography, phenyl-Sepharose column chromatography, DEAE-TSK column chromatography, and Reactive Red gel filtration 708960 3.4.22.53 DEAE-Sepharose CL-6B column chromatography 717157 3.4.22.53 DEAE Sephacel gel filtration 717623 3.4.22.53 HisTrap column chromatography 732375 3.4.22.53 single-step purification of calpain-1, calpain-2, and calpastatin using anion-exchange chromatography 754631