3.4.21.77 - 647582, 647587, 647588, 647594, 696922, 699819, 707359, 707618, 707727 3.4.21.77 by centrifugation and gel filtration 708777 3.4.21.77 by centrifugation, fractional precipitation by ammonium sulphate, ion-exchange chromatography and gel filtration, purified to homogeneity 699063 3.4.21.77 by gel filtration 696026, 707822 3.4.21.77 by HPLC 681472 3.4.21.77 by lysozyme, sonication, and centrifugation, His-tagged PSA solublized from inclusion bodies by urea and purified on nickel-chelate resin. GST-fused PSA solublized from inclusion bodies by sarcosyl and purified on glutathione agarose beads 681362 3.4.21.77 by thiophilic absorption chromatography 699826 3.4.21.77 fast purification method with 90% of purity and 50% of recovery 666798 3.4.21.77 free and complexed PSA, by gel filtration and immunoadsorption 710463 3.4.21.77 from LNCaP cell culture medium 647590 3.4.21.77 from seminal plasma 701037 3.4.21.77 native active enzyme from seminal plasma by immunoaffinity chromatography and anion-exchange chromatography 732851 3.4.21.77 nickel-nitrilotriacetic acid-Sepharose column chromatography and benzamidine-Sepharose column chromatography 680680 3.4.21.77 one third of the purified protein is enzymatically inactive, due to carboxy-terminal cleavage of Lys145 647580