3.4.18.1 - 3.4.18.1 purified 9.48fold via a one-step purification process using a DEAE-Sephagel HPLC column 3.4.18.1 recombinant enzyme from Pichia pastoris by cation exchange chromatography 3.4.18.1 recombinant GST-tagged enzyme from Escherichia coli strain BL21 by glutathione affinity and anion exchange chromatography