3.1.13.1	-	134050, 134061, 682697	
3.1.13.1	100fold	134057	
3.1.13.1	200fold	134054	
3.1.13.1	28.6fold	134055	
3.1.13.1	30fold	134049	
3.1.13.1	370fold, nearly to homogeneity	134058	
3.1.13.1	5300-26500fold, to homogeneity	134063	
3.1.13.1	all mutants, with the exception of R500K, by histidine affinity chromatography and the AKTA fast protein liquid chromatography system	709032	
3.1.13.1	anion exchange chromatography at denaturing conditions, in some cases followed by isoelectric focusing and dye binding chromatography	666317	
3.1.13.1	by affinity chromatography	700000	
3.1.13.1	by centrifugation, ion exchange and hydrophobic interaction chromatography	709993	
3.1.13.1	by histidine affinity chromatography and an AKTA HPLC system	707484	
3.1.13.1	Escherichia coli strain BL21(DE3)	664891	
3.1.13.1	full-length protein and mutants purified by affinity Ni-NTA chromatography, followed by ion exchange chromatography and gel filtration. 242-1001 construct purified to homogeneity	700205	
3.1.13.1	includes glutathione-Sepharose resin chromatography	656784	
3.1.13.1	partial purification that includes DEAE-Sepharose chromatography	656668	
3.1.13.1	partial purification, 500-700fold	134053	
3.1.13.1	partial purification, between 50-100fold	134051	
3.1.13.1	purification includes M2-agarose affinity resin	656862	
3.1.13.1	purified by inmunoprecipitation	657199	
3.1.13.1	purified on protein A-agarose and affinity GST-AtRrp4p inmobilized on nitrocellulose	656879	
3.1.13.1	recombinant	682083	
3.1.13.1	recombinant His- or FLAG-tagged wild-type non-acetylated and acetylated and mutants from Escherichia coli strain BL21(DE3) by affinity chromatography	751728	
3.1.13.1	recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography	750548	
3.1.13.1	recombinant RC-RNase 2 refolded from Escherichia coli strain BL21(DE3) inclusion bodies, further purification of the dialyzed enzyme by two different steps of cation exchange chromatography. The native enzyme is purified from oocytes by ultracentrifugation, extraction of the yolk granules from the pellet, followed by cation exchange chromatography	749739	
3.1.13.1	RNase R and RNase II constructs, full-length wild-type RNase R and RNase R mutant D278N	698974	
3.1.13.1	Rrp44-exosome (RE) architecture suggests an active site sequestration mechanism for strict control of 3' exoribonuclease activity in the RE complex	682555	
3.1.13.1	to apparent homogeneity, 270fold	134052	
3.1.13.1	wild-type and RNase II mutants purified by affinity chromatography	693048