2.7.2.7 - 2.7.2.7 ammonium sulfate precipitiation, heparin column chromatography, and Sephadex G25 gel filtration 2.7.2.7 HisTrap column chromatography 2.7.2.7 Ni-NTA resin column chromatography 2.7.2.7 nickel affinity column chromatography 2.7.2.7 partial